Applied Biophysics for Drug Discovery

<p><i>Applied Biophysics for Drug Discovery</i> is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. </p> <p>This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR.</p> <p>Topics covered include:</p> <p>•          Design considerations in biophysical-based lead screening</p> <p>•          Thermodynamic characterization of protein-compound interactions</p> <p>•          Characterizing targets and screening reagents with HDX-MS</p> <p>•          Microscale thermophoresis methods (MST)</p> <p>•          Screening with Weak Affinity Chromatography</p> <p>•          Methods to assess compound residence time</p> <p>•          1D-NMR methods for hit identification</p> <p>•          Protein-based NMR methods for SAR development</p> <p>•          Industry case studies integrating multiple biophysical methods</p> <p>This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.</p>