Assay Development

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Fundamentals and Practices

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ISBN: 9780470191156 Category:

<p>Essential principles and practice of assay development</p> <p>The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.</p> <p>Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:</p> <ul> <li> <p>The basics of assay development, including foundational concepts and applications</p> </li> <li> <p>Commonly used instrumental methods for both biochemical assays and cell-based assays</p> </li> <li> <p>Assay strategies for protein binding and enzymatic activity</p> </li> <li> <p>Cell-based assays</p> </li> <li> <p>High-throughput screening</p> </li> </ul> <p>An in-depth study of the now popular Caliper’s off-chip kinase assay provides an instructive, real-world example of the assay development process.</p>

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