Bioanalytics

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids.<br> <br> The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird’s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. <br> <br> The following techniques are covered in detail:<br> <br> – Purification and determination of proteins<br> – Measuring enzymatic activity<br> – Microcalorimetry<br> – Immunoassays, affinity chromatography and other immunological methods<br> – Cross-linking, cleavage, and chemical modification of proteins<br> – Light microscopy, electron microscopy and atomic force microscopy<br> – Chromatographic and electrophoretic techniques<br> – Protein sequence and composition analysis<br> – Mass spectrometry methods<br> – Measuring protein-protein interactions<br> – Biosensors<br> – NMR and EPR of biomolecules<br> – Electron microscopy and X-ray structure analysis<br> – Carbohydrate and lipid analysis<br> – Analysis of posttranslational modifications<br> – Isolation and determination of nucleic acids<br> – DNA hybridization techniques<br> – Polymerase chain reaction techniques<br> – Protein sequence and composition analysis<br> – DNA sequence and epigenetic modification analysis<br> – Analysis of protein-nucleic acid interactions<br> – Analysis of sequence data<br> – Proteomics, metabolomics, peptidomics and toponomics<br> – Chemical biology<br>