Molecular Biology Problem Solver

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. <p><b><i>Molecular Biology Problem Solver: A Laboratory Guide</i></b> asks the reader to consider crucial questions, such as:</p> <ul> <li>Have you selected the most appropriate research strategy?</li> <li>Have you identified the issues critical to your successful application of a technique?</li> <li>Are you familiar with the limitations of a given technique?</li> <li>When should common procedural rules of thumb not be applied?</li> <li>What strategies could you apply to resolve a problem?</li> </ul> <p>A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book’s expert contributors include:</p> <ul> <li>Working safely with biological samples and radioactive materials</li> <li>DNA and RNA purification</li> <li>PCR</li> <li>Protein and nucleid acid hybridization</li> <li>Prokaryotic and eukaryotic expression systems</li> <li>Properly using and maintaining laboratory equipment</li> </ul>